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Effect of different inducers and culture conditions on the expression of β2-toxin gene from Clostridium perfringens Type C
Pages: 1822-1827
Year: Issue:  10
Journal: Acta Agriculturae Zhejiangensis

Keyword:  Clostridium perfringene type Cβ2-toxinlactose;
Abstract: 对产气荚膜梭菌(Clostridium perfringens)β2毒素基因的表达载体pETXB2进行限制性核酸内切酶酶切鉴定,并用SDS-PAGE检测了不同培养条件下,该毒素基因在埃希氏大肠杆菌(Escherichia coli) BL21(DE3)工程菌株中的表达情况.结果表明,pETXB2重组质粒中的β2毒素基因位于pET-28c(+)表达盒中,且阅读框架和基因序列正确.以IPTG为诱导剂时,重组菌株BL21(DE3)(pETXB2)的最佳培养条件为37℃,pH7.0,IPTG终浓度0.8 mmol· L-,诱导4h;以乳糖为诱导剂时,其最佳培养条件为37℃,pH 7.0,乳糖终浓度0.5 g·L-1,诱导5h.
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