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Pages: 1061-1070
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Scapharca broughtoniigalectingene expressionimmune response;
Abstract: Galectins are a family of β-galactoside-binding lectins that play important roles in innate immunity of both vertebrates and invertebrates. In the present study, the c DNA of a galectin with multiple carbohydrate recognition domains(CRDs) was cloned from ark shell Scapharca broughtonii(designated Sb Gal) by partial c DNA sequence selected from the transcriptome library by rapid amplification of c DNA ends(RACE) technique. The full length c DNA of Sb Gal, another specific galectin in bivalve, was 3120 bp in length encoding a polypeptide of 554 amino acids, and contained four carbohydrate-recognition domains. The predicted molecular weight was 63.3158 k Da and the theoretical isoelectric point was 4.99. The results of multiple sequences alignment and phylogeny analysis show that the identity of Sb Gal shared with Crassostrea viginica, Pinctada fucata, and Argopecten irradians was 70%, 65%, and 46%, respectively. And the four domains of Sb Gal shared 36%—41% identities with each other. Quantitative Real-time PCR(q RT-PCR) was used to assess the m RNA distribution in all tested tissues, including hemocytes, foot, adductor muscle, mantle, gill, and hepatopancreas. The highest expression level was observed in hemocytes, while the lowest in hepatopancreas. After being challenged by Vibrio anguillarum, the relative m RNA expression level of Sb Gal was up-regulated significantly(P<0.01). The timing of maximum expression occurrence and returning to the initial level were different among the tissues. Therefore, Sb Gal expression is of obvious time dependence and instantaneous expression pattern, and it could function as a pattern recognition receptor in immune defense of ark shells.
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