The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
RELATIVE EXPRESSION OF GENES HSP70 AND HSP90 IN SEA URCHIN STRONGYLOCENTROTUS INTERMEDIUS IN THERMAL STRESS
Author(s): 
Pages: 1034-1039
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Strongylocentrotus intermediushsp70hsp90temperatureRT-PCR;
Abstract: To understand the response behavior of heat shock proteins(hsp70 and hsp90) under thermal stress in sea urchin Strongylocentrotus intermedius, we determined the relative expression quantity of hsp70 and hsp90 in adult urchin tissues(including tube-feet, coelomocytes, intestine, gonad and lantern using the RT-PCR(reverse transcription polymerase chain reaction). Different expressions of the two genes between thermal-tolerant chains and control ones were compared especially in tube feet. The results show that the two genes are expressed ubiquitously in various adult tissues and responded quickly to the thermal stress. Relative expressions of the genes in tube feet, coelomocytes, and intestine of sea urchins reached their highest levels in 6h(29°C), which is significantly higher than the control points(P<0.05). The relative expression of hsp70 and hsp90 reached the maximum in 12h(24°C) and 24h(22°C) respectively in gonads. Relative expression of the two genes reached maximum at 12 h in lantern. In tube feet and coelomocytes, the expression trends of hsp70 and hsp90 were quite similar and no significant difference was found in different time(P>0.05). The expression of hsp70 increased gradually from the beginning of experiment, then decreased despite of temperature variation, and no significant difference between heat-resistant and control sea urchin chains at all the time points(P>0.05). In heat-resistant chains, the expression of hsp90 increased at first, and then decreased gradually at 12 h, but the expression of hsp90 in the two heat-resistant chains were higher than the two control ones(P<0.05). The result indicates that thermal stress can significantly affect the expression of hsp90; and therefore, hsp90 can be used as an internal thermal-tolerant indicator in heat-resistant selection breeding of S. intermedius.
Related Articles
No related articles found