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MOLECULAR CLONING AND FUNCTIONAL ANALYSIS OF TWO DIFFERENT Wap65 GENES FROM SCOMBEROMORUS NIPHONIUS
Author(s): 
Pages: 1052-1060
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Scomberomorus niphoniusWap65gene cloningexpressionfunction research;
Abstract: Full-length c DNA sequences of Wap65-1 and Wap65-2 were cloned first time from the Scomberomorus niphonius by homology cloning and RACE techniques. The Wap65-1 and Wap65-2 sequences are 1659 bp and 1725 bp, encoding 426 and 436 amino acids respectively. Sequences comparison and phylogenetic analysis showed that the homology of Wap65-1 and Wap65-2 is 60%, which located in different branches. Furthermore, the fish larva were induced by high temperature, the real-time quantitative PCR results showed that the expression of two different genes are up-regulated under the high temperature induced, and the expression of Wap65-2 gene show significant difference(P<0.05). On this basis, we succeeded getting the soluble expression of two proteins by contracting two kinds of Cold-Shock expression system, and found Wap65-2 had better strong effect on survival protection under high temperature stress. The study laid a theoretical foundation for breeding heat resistant varieties of S. niphonius.
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