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OPTIMIZATION OF CARBON RESOURCE FOR MACROLACTIN A PRODUCTION FROM MARINE BACILLUS AMYLOLIQUEFACIEN
Author(s): 
Pages: 1071-1077
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  high-speed counter-current chromatography(HSCCC)Bacillus amyloliquefaciensMacrolactin Acarbon resource optimization;
Abstract: To optimize the production of Macrolactin A from a marine Bacillus amyloliquefaciens ESB-2, we extract crude material with macroporous adsorptive resin and then purified in high-speed counter-current chromatography(HSCCC). The purity of separated component was determined and the influence of different carbon sources on Macrolactin A yield was detected, in high performance liquid chromatography(HPLC). The fermentation curve of marine Bacillus amyloliquefaciens was obtained under optimization condition. The results show that Macrolactin A could be purified quickly in HSCCC, and the purity of the product reached >95%. The carbon sources of glucose, sucrose, and maltose were conducive for the accumulation of Macrolactin A, and maltose was the best carbon source at concentration of 1%, under which the concentration of Macrolactin A could reach 18.5 mg/L and the production was more than doubled.
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