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Pages: 1109-1118
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Vibrio anguillarumphysiological and biochemical identification16S rRNA geneserotypingantibiotics sensitivity;
Abstract: Vibrio anguillarum is a bacterial pathogen commonly found in fish mariculture. There are 23 serotypes assigned for V. anguillarum, and O1, O2, and O3 serotype are associated with fish vibriosis. At present, few is known about the serotype and antibiotics resistance of V. anguillarum from mariculture fish in China. The present studies were carried out to reveal the serotype and antibiotics resistance of 31 clinical V. anguillarum strains isolated from fish farm. API ID 32 E identification system was used to analyze the physiological and biochemical characteristics of the tested strains. The results showed the strains had the constant and variable physiological and biochemical characteristics, and thus was difficult to identify these strains to species level based on the physiological and biochemical characteristics. Nevertheless, the 16 S r RNA gene sequences of the isolates revealed the highest identities(99%—100%) with V. anguillarum strains deposited in the Gen Bank, and phylogenetic tree analysis based on 16 S r RNA gene sequences showed that all isolates concentrated in a cluster with known V. anguillarum strains. Therefore, all clinical isolates could be identified as V. anguillarum. Moreover, serotypes of the V. anguillarum isolates were determined by glass agglutination reaction test using rabbit anti-serum against O1—O5 serotype V. anguillarum. The results displayed that the isolates belonged to O1, O2, O3, and O5 in number of 16, 5, 3, and 1, respectively, presenting three potential pathogenic serotypes(O1—O3) of V. anguillarum strains, of which O1 was prevalent. Furthermore, the antibiotics sensitivity of V. anguillarum against 28 antibiotics were detected with ATB system. The results show that the resistance of the isolates reached 100% against seven antibiotics(lincomycin, fusidine, metronidazole, amoxicillin, amoxicillin/clavulanic acid, oxacillin, penicillin) and 94.4%(34 strains) against three antibiotics(erythromycin, pristinamycin, tylosin). Over 83%(30 strains) were resistant against 12 antibiotics. Therefore, most V. anguillarum isolates were multi-antibiotics-resistant strains. The findings here may help scientists to investigate the epidemiology of V. anguillarum and to control fish vibriosis.
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