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ACE INHIBITORY PEPTIDE IN SQUID LIVER HYDROLYSATE: SEPARATION, PURIFICATION, AND BIOACTIVITY ANALYSIS
Author(s): 
Pages: 1175-1179
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  squid liver wasteACE inhibitory peptideseparation and purificationbioactivity analysis;
Abstract: For make use of leftover from squid industry, we separated and purified the inhibiting peptide from squid liver and studied its bioactivity by hydrolyzing squid liver protein. Enzymolysis and gel filtration separation technology were applied. Results show that after the enzyme solution was ultra-filtrated(molecular weight 20 k Da), five components were separated on Sephadex G-100. Among them, Component 2 displayed a high ACE(angiotensin converting enzyme)-inhibitory activity with half-inhibitory concentration(IC50) at 1.92mg/m L. In addition, the ACE inhibitory activity remained largely no change in temperature of 0—100 °C and 1—12 in p H. Further purification on Component 2 in DEAE anion exchange column on Sephadex LH-25, additional two components 8 and 9 in IC50 of 1.58 and 2.12 mg/m L, respectively. Therefore, the result may offer a reference to research and develop medicines for high-blood pressure treatment.
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