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MOLECULAR CLONE AND EXPRESSION OF C-TYPE LECTIN IN MERETRIX MERETRIX
Author(s): 
Pages: 1186-1192
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Meretrix meretrixC-type lectingene clonebioinformaticsenvironmental factors;
Abstract: The C-type lectin is one of the most important pattern recognition receptors. In present study, a C-type lectin was cloned from Meretrix meretrix(Mm-CTL) for the first time using degenerated PCR and rapid amplification of complementary DNA ends(RACE) method. The full length of the Mm-CTL c DNA was 1855 bp containing a 519-bp open reading frame(ORF) encoding a 172-amino-acid polypeptide. Prediction of protein domains revealed that the Mm-CTL contain a typical carbohydrate recognition domain(CRD) that ranged 34 th to 168 th amino acid. BLASTP analysis showed that the Mm-CTL shared 56.5% identity with the ortholog in Solen grandis, and 13.5%—23.9% with other invertebrate orthologs. Mm-CTL is distinctly clustered with mollusk as expected in phylogenetic analysis. After Vibrio alginolyticus challenge, the Mm-CTL m RNA was induced significantly in 6h post challenge, and its expression peaked in 12 h. Moreover, the Mm-CTL expression reduced in salinity 5 compared to that in salinity 10—20. In a thermal stress, the Mm-CTL expression was down-regulated at 10°C, whilst increased at 35°C. Therefore, the expression of Mm-CTL might response to bacterial infections or environmental stress. The findings may help understanding innate immune factor in molluscs for disease prevention.
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