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CLONING AND BIOINFORMATICS ANALYSIS OF EPO GENE IN ANTARCTIC ICEFISH CHIONODRACO HAMATUS AND TREMATOMUS BERNACCHII
Author(s): 
Pages: 1193-1201
Year: Issue:  5
Journal: Oceanologia et Limnologia Sinica

Keyword:  Antarctic icefishEPO genegene cloningadaptive evolutionbioinformatics;
Abstract: We used PCR(polymerase chain reaction), TA(based on thymine(T) and adenine(A)) cloning, and nextgeneration sequencing technology to isolate genomic DNA sequences of EPO(erythropoietin) gene from healthy Chionodraco hamatus and Trematomus bernacchii, and then we used bioinformatics methods to analyze their genetic characteristics, such as genetic structure and conserved domain. The EPO gene of C. hamatus and T. bernacchii are 2727 bp and 2820 bp, respectively. Both of them contain five exons and four intron structures, and the coding region is 558 bp in length, encoding 185 amino acids. In addition, EPO of both fish are a relatively soluble and acidic protein. The tertiary structure prediction shows a typical structure characteristic that they fold into a four-parallel-α-helix structure, which assue EPO keep original function in the organism. Amino acids alignment shows 95.2% identity in homology of the EPOs between C. hamatus and T. bernacchii. Compared with other species, the EPOs shared 53.9%—85.5% identities. The phylogenetic tree of the EPO nucleic acid sequences shows a close relationship among C. hamatus, C. hamatus and other fishes. The phyletic evolution agrees with their genetics, showing a conservative evolution progress of the EPO gene. This study may provide a theoretical basis for revealing the genetic characteristics of the Antarctic icefish EPO gene, and lay foundation to EPO study on long-term biological adaption under an extremely low temperature and high oxygen environment.
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