BACTERIAL DIVERSITY AND COMMUNITY STRUCTURE IN SEDIMENTS OF XIXI WETLAND, HANGZHOU
Author(s): HUANG Yuan, FANG Xu, CHU Wen-Ke, CHEN Min, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang Institute of Microbiology
Pages: 1202-1209
Year: 2015 Issue: 5
Journal: Oceanologia et Limnologia Sinica
Keyword: Xixi wetland; sediment; bacterial diversity; MiSeq high-throughput sequencing;
Abstract: The Illumina Mi Seq sequencing-based analysis of the V3-V4 16 S r RNA gene region was used to detect bacterial diversity and community structure in Xixi wetland, Hangzhou. Overall, 67734 optimized reads with an average length of 441 bp were obtained from four sediment samples. Bacterial diversity was very high in these samples, since a total of 2181 OTUs were identified which could be assigned into 30 different phyla and 252 genera. The dominant group belonged to Proteobacteria, accounting for 31.0%—64.7% of total sequences. Among Proteobacteria, the beta-, delta- and gamma-subdivisions were dominant classes. Chloroflexi, Firmicutes and Bacteroidetes were also very common, accounting for 7.0%—43.0%, 1.1%—14.7% and 1.0%—10.2% of total libraries, respectively. The ordination analysis showed that there were about 10%—15% of total sequences belonged to the unclassified group, which meant that there may be many undiscovered species in sediments. There were also large number of DNA sequences affiliated to the strains which related to N and S metabolism in beta-, gamma-, and delta-Proteobacteria branches. This study will lay the foundation for future research on microbiology in Xixi wetland.
Pages: 1202-1209
Year: 2015 Issue: 5
Journal: Oceanologia et Limnologia Sinica
Keyword: Xixi wetland; sediment; bacterial diversity; MiSeq high-throughput sequencing;
Abstract: The Illumina Mi Seq sequencing-based analysis of the V3-V4 16 S r RNA gene region was used to detect bacterial diversity and community structure in Xixi wetland, Hangzhou. Overall, 67734 optimized reads with an average length of 441 bp were obtained from four sediment samples. Bacterial diversity was very high in these samples, since a total of 2181 OTUs were identified which could be assigned into 30 different phyla and 252 genera. The dominant group belonged to Proteobacteria, accounting for 31.0%—64.7% of total sequences. Among Proteobacteria, the beta-, delta- and gamma-subdivisions were dominant classes. Chloroflexi, Firmicutes and Bacteroidetes were also very common, accounting for 7.0%—43.0%, 1.1%—14.7% and 1.0%—10.2% of total libraries, respectively. The ordination analysis showed that there were about 10%—15% of total sequences belonged to the unclassified group, which meant that there may be many undiscovered species in sediments. There were also large number of DNA sequences affiliated to the strains which related to N and S metabolism in beta-, gamma-, and delta-Proteobacteria branches. This study will lay the foundation for future research on microbiology in Xixi wetland.
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