Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci
Author(s): DONG Yan, LIN Shuang-shuang, CAO Yu, WU Wei-wei, HUANG Shu-qin, ZHENG Wei-guo, LI Fa-yuan, GE Bin-wen, GUO Yu-lin, ZHOU Huai-gu
Pages: 373-376,380
Year: 2015 Issue: 5
Journal: Journal of Forensic Medicine
Keyword: forensic genetics; reagent kits; short tandem repeat sequences; autosom e; Y chrom osom e;
Abstract: Objective To establisha multiplex STR genotyping m ethod for autosom al STR and Y-STR loci in forensic biological practice. Methods W idely used autosom al STR loci and Y-STR loci w ere se-lected. A set of PC R prim ers w as designed, and a 5-dye fluorescent labeled STR multiplex PC R reagent kit w as developed. Results A kit w as developed w hich can sim ultaneously detect 15 autosom al STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosom al STR plus 10 Y-STR kit in com bination w ith capillary electrophoresis m ethod w as used to STR genotyping w ith accurate and reli-able results. The new one-step testing kit can potentially be w idely used in forensic cases and D N A databank in the future.
Pages: 373-376,380
Year: 2015 Issue: 5
Journal: Journal of Forensic Medicine
Keyword: forensic genetics; reagent kits; short tandem repeat sequences; autosom e; Y chrom osom e;
Abstract: Objective To establisha multiplex STR genotyping m ethod for autosom al STR and Y-STR loci in forensic biological practice. Methods W idely used autosom al STR loci and Y-STR loci w ere se-lected. A set of PC R prim ers w as designed, and a 5-dye fluorescent labeled STR multiplex PC R reagent kit w as developed. Results A kit w as developed w hich can sim ultaneously detect 15 autosom al STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosom al STR plus 10 Y-STR kit in com bination w ith capillary electrophoresis m ethod w as used to STR genotyping w ith accurate and reli-able results. The new one-step testing kit can potentially be w idely used in forensic cases and D N A databank in the future.
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