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Establishment of PEGylated decellularized valves modified by vascular endothelial growth factor in vitro
Pages: 2481-2484
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  Polyethylene glycolVascular endothelial growth factorMichael addition reactionDecellularized valveScaffold;
Abstract: Objective We established decellularized valvemodified by vasculaendothelial growth facto(VEGF) in vitro based on michael addition reaction, and explored theimechanical and biological properties.Method4-arm-polyethylene glycol (PEG)-acrylate waused to PEGylate the decellularized aortivalves.The PEGylated decellularized valvewere modified by VEGF in vitro.The establishmenresulof composite valvewaobserved.Mechanical propertieof valvewere tested acircumferential and radial directions.Theibiological propertiewere measured by DSC, hemolytiand cytotoxicity test.ResultWe confirmed thaPEGylated decellularized valvewere successfully modified by VEGF viFourietransform infrared spectroscopy and immunofluorescence.The decellularized resultshowed collagen fiberthickened in alignment.MechanicPerformance Testing showed thaPEGylated valvecould improve parmechanical properties.DSdisplayed denaturation temperatureof DPAV, PEG-DPAV and VEGF-PEG-DPAV were (65.64 ± 0.15) ℃, (74.94 ± 0.10) ℃ and (74.88 ± 0.05) ℃ respectively.Hemolytitesshowed hemolysiratiowere (0.23 ±0.01)%, (0.24 ± 0.01)% and (0.23 ± 0.01) % , respectively.No cytotoxicity waobserved in DPAV, PEG-DPAV and VEGF-PEG-DPAV.Conclusion We successfully established PEGylated decellularized valvemodified by VEGF, which provided feasible method in scaffold modification of decellularized valvein vitro.
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