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The research of the mechanism of tumor suppressor in lung cancer-1 gene on the Invasion and metastasis of Hepatocellular carcinoma 7402 cells
Pages: 2347-2349
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  Tumor suppressor in lung cancer-1Human hepatocellular carcinomaAdhesionInvasion and metastasis;
Abstract: Objective To study the effecof tumosuppressoin lung cancer-1 (TSLC-1) gene transfected into hepatocellulacarcinomcell line 7402, and to explore the mechanism of TSLC-1 gene on the metastaseof human hepatocellulacarcinom(HCC) cells.MethodThe recombinanplasmid of pcDNA3.1 hisC-TSLC-1 wastably transfected into 7402 cell.The effecto 7402 gene on the homogeneity adhesion and the heterogeneity adhesion of hepatocellulacarcinomcellwere measured with cell count, The in vitro migration of pcDNA3.1hisC-TSLC-1 and mock cellon extrcellulamatrix natrigel waassayed by monolayewound healing test.ResultThe normalized initial cDNconcentration of TSLC-1 in L02, MHCC97-L and MHCC97-H were (33.15 ± 0.05) × 103, (9.06 ± 0.03) × 103,(6.14 ±0.09) × 103, respectively.There wasignificandifference in expression level in livecancecell strainwith differenmetastatipotential.The homogeneity adhesion of 7402-TSLC-1 cell group (1 301 ± 24) wahighethan empty vectogroup (1 279 ± 20) and contrasgroup (987 ± 26), and the heterogeneity adhesion walowethan empty vectogroup and contrasgroup.The in vitro cell adhesion experimenshowed thathe exogenouTSLC-1 gene in the 7402-TSLC-1 celldecreased cell adhesion to matrigel and fibnectin extrcellulamatrix by 49.3% and 37.5%, respectively, compared with the control cells.The migration of the 7402-TSLC-1 cellon the matrigel waraised by 31.5%.Conclusion The human imprinted gene TSLC-1 can reduce homogeneity adhesion, promote heterogeneity adhesion and migration of 7402-TSLC-1 cells.TSLC-1 gene mighfunction ametastasisuppressogene foHCC.
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