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Oroxylin A inhibits cell proliferation and induces apoptosis of MHCC97-H cells by regulating AMP-activated protein kinase signaling pathway
Pages: 2392-2394
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  HepatomaOroxylin AProliferationAMP-activated protein kinase;
Abstract: Objective To investigate the effectof oroxylin on cell proliferation and apoptosiof hepatomcellMHCC97-H and demonstrate the moleculamechanism underlying effectof oroxylin A.MethodCell proliferation waexamined by methyl thiazol tetrazolium (MTT) assay.The apoptosirate waevaluated by propidium iodide (PI)/Annexin Ⅴ-fluoresceine isothiocyanate (FITC) double staining method.The protein levelof AMP-activated protein kinase (AMPK) were examined by Western blotting,respectively.Result50, 100 and 150 μmol/L oroxylin inhibited cell proliferation of MHCC97-H cellin concentration-dependenmanner.The apoptosirate of MHCC97-H cellexposed to 50, 100 and 150 μmol/L oroxylin wa(18.87 ± 0.37)%, (20.93 ± 0.32)% and (24.37 ± 0.47)%, respectively.100 and 150 μmol/L oroxylin caused decreased accumulation and phosphorylation of AMPK in MHCC97-H cells.Conclusion Oroxylin inhibitcell proliferation and induceapoptosiof MHCC97-H cellby regulating AMPK signaling pathway.
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