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Experimental study of bufalin on inhibiting cell proliferation and apoptosis in liver cancer cells with high metastatic potential
Author(s): 
Pages: 2388-2391
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  BufalinCancerliver cellProliferationApoptosis;
Abstract: Objective To investigate the inhibitory effectof Bufalin on human hepatocellulacarcinomcellwith high metastatipotential.MethodThe human hepatomcellwith high metastatipotential (HCC-LM3) were cultured in vitro.The bufalin warespectively added into culture fluid with variouconcentration of 0.02, 0.04, 0.08 mg/L.The 5-fluorouracil (5-Fu) warespectively added into culture fluid with variouconcentration of 1.05, 12.34, 25.79 mg/L.The cell growth, proliferative cycle and apoptosiwere respectively determined by cell counting kit-8 (CCK-8) assay and flow cytometry technique a24, 48, 72 h.ResultThe growth inhibitory rate of HCC-LM3 cellgradually increased with the increase of bufalin concentration athe same time (F =14.823, 15.623, 4.737, P < 0.05) owith the time extension of bufalin athe same concentration (F =44.757, 43.256, 19.682, 17.112,50.765, 31.214, P < 0.05).Compared with control group, the cell percentage of HCC-LM3 cellaG1 phase gradually decreased with the increase of bufalin concentration[IC50 : (43.96 ± 3.52) % vs.(70.19 ± 4.70) %, IC70 : (40.99 ± 5.72) % vs.(70.19-± 4.70) %, F =23.924, P < 0.05], and the cell percentage of HCC-LM3 cellaG2 pluphasegradually increased with the increase of bufalin concentration [IC50:(56.04±3.52)% vs.(29.81 ±4.70)%,IC70:(59.01 ±5.72)% vs.(29.81 ±4.70)%,F=23.924, P < 0.05].The apoptotirate of HCC-LM3 cellgradually increased with the increase of bufalin concentration avarioutime (F =6.817, 46.639, 77.658, P < 0.05).The apoptotirate of HCC-LM3 cellinduced by bufalin wahighethan thaof 5-Fu a48 h and 72 h [IC50 : (34.01 ± 4.62) % vs.(22.02 ±1.29)%,IC70:(47.60±4.41)% vs.(31.90 ±4.95)%,t=-6.381,P<0.05] and 72 h [IC50:(52.01±6.63)% vs.(32.65 ±3.69)%,IC70:(74.31 ±6.18)% vs.(47.85 ±4.52)%,=-5.729,P < 0.05].Conclusion Iione of the importanmechanismof bufalin in inhibiting the growth of human hepatocellulacarcinomcellwith high metastatipotential by blocking the cell proliferation aG2 pluphaseand inducing the cell apoptosis.
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