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Liver regeneration phosphatase 3 regulates the aerobic glycolysis of colon cancer cells
Pages: 2448-2449
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  Liver regeneration phosphatase 3Colon cancerAerobic glycolysis;
Abstract: Objective To investigate whetheliveregeneration phosphatase 3 (PRL-3) participatein the regulation of colon cancecell metabolism, and enhancethe aerobiglycolysis.MethodPRL-3 wastable transfected into LoVo cells, cell (1 × 106) medium wacollected to detecthe level of lactate acid.Besides, real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blowaused to detecthe expression of related enzymeof glycolysiin LoVo cell(1 × 106).ResultLoVo cellwhich were transfected with PRL-3 (LoVo-P) expressed more lactate than LoVo celltransfected with Vector-Control (LoVo-C) and LoVo cell[(11.9 ± 2.7) mol/L vs.(4.2 ± 1.6) mol/L vs.(3.7 ± 1.2) mol/L, P < 0.05].Besides, RT-qPCrevealed thaphosphorylatepyruvate kinase M2 (PKM2), glucose transporte1 (Glut1), pyruvate dehydrogenase (PDH) increased in LoVo-P, which are the key enzyme in glycolysis.Wrstern bloalso tested the same resultin the protein level.Conclusion PRL-3 regulateLoVo cellmetabolism and enhance the aerobiglycolysis, in thiwav LoVo cellcan betteadapthe tumomicroenvironment.
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