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Effects of type Ⅳ collagenase on proliferation, migration and invasion of glioma
Author(s): 
Pages: 2456-2458
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  Type Ⅳ collagenaseGliomaIn vitroMetastasis;
Abstract: Objective To study the expression of type Ⅳ collagenase in human gliomtissue, and the effectof type Ⅳ collagenase on proliferation, migration and invasion of Gliomcells, so ato provide basifoclinical treatment.MethodWestern blotting waused to compare the contenof type Ⅳ collagenase between malignanGliomtissueand normal tissues.Gliomcell C6 were divided into three groups, respectively added with cell culture medium (control group), type Ⅳ collagenase (study group),type Ⅳ collagenase and specifiinhibito(inhibitogroup).Use the methyl thiazol tetrazolium (MTT) assay, transwell migration and invasion assay to observe the effectof type Ⅳ collagenase on proliferation,migration and invasion of Gliomcells.ResultThe expression rate of type Ⅳ collagenase in malignangliomtissue(87.5%) ihighethan iin normal tissue(20.0%).There wasignificandifference in relative absorption ratio between two group(0.81 ±0.12 vs.0.04 ±0.01 ,P <0.01).The resulof MTassay indicate the cell proliferation ability of the three grouphave no statistical significance aevery point.The cell counin migration assary of study group (137.49 ± 14.53) wasignificantly more than control group (65.35 ±6.57, P<0.01) and inhibitogroup (67.42 ±7.92, P<0.01).Similarly, the cell counin invasion assary of study group (33.67 ± 7.47) wasignificantly more than control group (20.45±4.89, P<0.01) and inhibitogroup (21.67±5.83, P<0.01)Conclusion The contenand the expression rate of type Ⅳ collagenase in MalignanGliomtissueihighethan iin normal tissues.Type Ⅳ collagenase promotecell migration and invasion, and could be blocked by specifiinhibitor, providing new wayin the treatmenof malignanglioma.
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