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Interleukin-24 inhibits tumor cell metastasis through (C-X-C motif) ligand 12/(C-X-C motif) receptor 4 signal axis
Pages: 2465-2468
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  Nonsmall-cell lung cancerInterleukin-24(C-X-C motif) ligand 12/(C-X-C motif) receptor 4Metastasis;
Abstract: Objective To investigate whetheinterleukin-24 (IL-24) could inhibithe chemokine (C-X-motif) ligand (CXCL)-12/chemokine (C-X-motif) recepto(CXCR)-4 signaling pathway and suppreslung cancecell migration and invasion in vitro.MethodThe lung cancecell line H1299 wastably transfected with recombinanlentiviral vectorharboring open reading frame (ORF) of IL-24 and used in the presenstudy to determine the inhibitory effectof IL-24 on CXCL12/CXCR4 axis.The inhibitory effectof IL-24 on CXCL12/CXCR4 were assessed by Western blotting.Biological function wastudied using cell migration and invasion assays.ResultIL-24 expression in the H1299-IL24 cell line resulted in reduced CXCR4 expression on both mRNand protein level(P < 0.05).Functional studieshowed thaIL-24 inhibited tumocell migration (30% decrease) and invasion (30%-40% decrease, P <0.05).Finally, IL-24, when combined with CXCR4 inhibito(AMD3100), demonstrated enhanced inhibitory activity on tumocell migration (P < 0.05).Conclusion IL-24 interferewith the CXCL12/CXCR4 signaling pathway and inhibitlung tumocell migration and invasion.Additionally,IL-24, when combined with CXCR4 inhibitor, exhibited enhanced anti-metastatiactivity.
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