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Effects of sevoflurane pre-and post-conditioning on hypoxia/reoxgenation-induced injury of neonatal rat cardiomyocytes cultured in high-glucose culture medium and the role of glycogen synthase kinasease-3β
Author(s): 
Pages: 2477-2480
Year: Issue:  10
Journal: Chinese Journal of Experimental Surgery

Keyword:  SevofluraneGlycogen synthase kinase-3βReperfusion injuryPreconditioningPostconditioning;
Abstract: Objective To evaluate the effecof sevoflurane pre-and post-conditioning on hypoxia/reoxgenation-induced injury of neonatal racardiomyocytecultured in high-glucose culture medium and the role of glycogen synthase kinase-3β.MethodPrimary cultured neonatal ra(1-3 d aftebirth) cardiomyocytewere randomly divided into 12 groups: Normal groupuse low-glucose (concentration,5.56 mmol/L) DMEM medium, hyperglycemigroupuse high-glucose (concentration, 25 mmol/L)DMEM medium.Control group (group NC/HC);hypoxia/reoxgenation group (group NH/R/HH/R);sevoflurane postconditioning group (group NS-Post/HS-Post);sevoflurane pre-and post-conditioning group (group NS-Pre + S-Post/HS-Pre + S-Post);SB216763 group (group NSB/HSB);Dimethyl sulfoxide group (group N-DMSO/H-DMSO).Cell survival rate, apoptotirate, the LDH activity and the levelof phosphor-GSK-3β were measured athe end of the experiment.ResultNH/R,HS-Post, HS-Pre + S-Posgroup were (52.4 ± 3.4) %, (61.1 ± 4.5) %, (71.2 ± 2.8) %, respectively.HS-Posgroup、HS-Pre + S-Posgroup reduced the apoptotirate compared with group NH/R.However, HH/R, HS-Post, HS-Pre +S-Posgroup were (48.2 5.1)%, (50.8 ±4.9)%,(48.5 ± 2.8) %, respectively.HS-Posgroup, HS-Pre + S-Posgroup didn' reduce the apoptotirate compared with group NH/R.Conclusion Cardioprotection induced by sevoflurane iabolished by hyperglycemia.GSK-3β may be involved with above processes.
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