Study on the mechanisms of Carassius auratus sialoglycoproteins in suppressing the differentiation of osteoclast
Author(s): YU Zhe, WANG Jing-feng, MAO Lei, ZHOU Xiao-chun, XUE Chang-hu, College of Food Science and Engineering, Ocean University of China
Pages: 33-40
Year: 2015 Issue: 5
Journal: Chinese Journal of Marine Drugs
Keyword: Ca-SGP; TRAP; NF-κB; MAPK;
Abstract: Objective To investigate the effects of Ca-SGP on the RANKL-induced osteoclastogenesis and signaling pathways.Methods Osteoclast was differentiated from mouse bone marrow-derived macrophages(BMMs)with M-CSF and RANKL.Osteoclast and its precursor activity were measured by MTT.Osteoclast numbers were evaluated by TRAP stains.mRNA levels were analyzed by qRT-PCR.Results Ca-SGP inhibited the osteoclast differentiation without toxicity,and reduced the generation of TRAPpositive multinucleated cells by suppressing MAPK and NF-κB signaling pathways.Conclusion Ca-SGP suppressed osteoclastogenesis through inhibiting RANKL-induced MAPKs and NF-κB pathway.
Pages: 33-40
Year: 2015 Issue: 5
Journal: Chinese Journal of Marine Drugs
Keyword: Ca-SGP; TRAP; NF-κB; MAPK;
Abstract: Objective To investigate the effects of Ca-SGP on the RANKL-induced osteoclastogenesis and signaling pathways.Methods Osteoclast was differentiated from mouse bone marrow-derived macrophages(BMMs)with M-CSF and RANKL.Osteoclast and its precursor activity were measured by MTT.Osteoclast numbers were evaluated by TRAP stains.mRNA levels were analyzed by qRT-PCR.Results Ca-SGP inhibited the osteoclast differentiation without toxicity,and reduced the generation of TRAPpositive multinucleated cells by suppressing MAPK and NF-κB signaling pathways.Conclusion Ca-SGP suppressed osteoclastogenesis through inhibiting RANKL-induced MAPKs and NF-κB pathway.
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