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Factors that influence intestinal microflora formation in larval turbot (Scophthalmus maximus L.)
Author(s): 
Pages: 1075-1083
Year: Issue:  5
Journal: Journal of Fishery Sciences of China

Keyword:  Scophthalmus maximus L.larva breedingintestinal tract microflorainfluence factorhigh-through sequencing technique;
Abstract: Turbot (Scophthalmus maximus) is a cold-water marine fish. In recent years, with the expansion and im-provement of turbot farming, bacterial disease has severely affected turbot development, especially in larvae. Because the fish have the special requirements for the living environment, disease occurrence is the result of interactions among fish, pathogens, and the environment. The gastrointestinal flora plays an important role in fish growth and development, and is closely related to disease occurrence. Therefore, it is necessary to investigate the gastrointestinal flora of larval fish. In this study, factors that influence gastrointestinal microflora formation and succession in turbot larvae were in-vestigated by high-throughput sequencing. The intestinal tract microflara and their relationships with different devel-opment stages (e.g., larvae and fertilized eggs), chlorella, rotifer, artemia, pellet feed, and tank inlet water were ana-lyzed. The results revealed that the intestinal tract microflora of turbot larvae was stable after initial feeding and was directly affected by bacteria in fertilized eggs. Meanwhile, microflora structure was stable during turbot larval devel-opment and barely influenced by the bacteria in water and feed. The dominant bacterium was Lactococcus piscium at a relative proportion of up to 45%–65%in larval intestines. In addition, the relative abundance of this bacterium in fertil-ized eggs reached 10.4%. Basing on these results, we suggest that the gastrointestinal tract of turbot larvae may be se-lective for the colonized bacteria. Some bacteria in water and feed, such as the Streptococcus sp., Pseudomonas sp., and Carnobacterium sp., can colonize intestines as subdominant strains.
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