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Determination of notoginsenoside K in Notoginseng total saponins by ultra performance liquid chromatography method
Author(s): 
Pages: 1072-1074
Year: Issue:  9
Journal: World Phytomedicines

Keyword:  Notoginseng total saponinsnotoginsenoside KUPLC;
Abstract: Objective To establish a method for determination of notoginsenoside K in Notoginseng total saponins by ultra performance liquid chromatography (UPLC) method.Methods The determination was carried out on Cosmosil Cholester column (50 mm × 2.0 mm, 2.5 μm). The mobile phase consisted of [water - acetonitrile (85∶15)] - acetonitrile with gradient elution at a flow rate of 0.5 mL/min. The detection wavelength was set 203 nm and the column temperature was at 30℃with injection volume of 2μL. Results Notoginsenoside K had good linearity in the ranges of 2.020 — 101.000μg/mL (r=0.999 9). The average recovery was 99.50% with RSD of 1.35%.Conclusion The method has simple operation, accurate results, good reproducibility, and specificity which can be used in the quantity control of notoginsenoside K in Notoginseng total saponins effectively.
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