Author(s): ZHANG Guibiao, SONG Ya’nan, Dong Su, LU Yiyu, SUN Mingyu, LIU Ping, SU Shibing, Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Institute for Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, E - Institute of Chinese Traditional Internal Medicine, Shanghai university of Traditional Chinese Medicine
Pages: 2103-2108
Year: 2015 Issue:  9
Journal: Chinese Archives of Traditional Chinese Medicine
Keyword:  HQD; YCHD; liver fibrosis; differentiated expressed genes; signaling pathway;
Abstract: Objective: To investigate the effects and molecular mechanism of Huangqi Decoction(HQD) and Yinchenhao Decoction(YCHD) on the liver fibrosis in rats through the comparative analysis of differential gene expression profiles and signaling pathways. Methods: DMN induced liver fibrosis model in rats were randomly divided into normal group,HQD group,YCHD group and model group,treated by HQD or YCHD. The pathological changes of hepatic tissue were observed and hydroxyproline(Hyp) contents were measured. The gene expression profile was detected by whole genome chip testing and the differentiated expressed genes(DEGs),gene ontology(GO) and signal pathways were analyzed. Results: Compared to normal group,model group presented the pathological changes of liver damage and liver fibrosis and the Hyp content of liver tissue significantly increased(P < 0. 01). Compared to model group,in HQD and YCHD group,the pathological changes were markedly improved of liver tissue and collagen hyperplasia degree and Hyp contents were significantly reduced(P < 0. 01) and the effects in YCHD was slightly better than in HQD. Compared to model group,the DEGs of HQD and YCHD were 373 and 432,including 189 co-own gene. Both of treatment group acted on Notch and Notch-induced EMT signaling pathway,and cell adhesion,cell matrix glycoconjugates pathway. HQD group still acted on VEGF and angiopoietin pathways,thrombospondin pathway and so on. YCHD group still acted on cell adhesion-ECM remodeling pathway,EMT regulation pathway and so on. Conclusion HQD and YCHD significantly improved liver fibrosis induced by DMN in rats. There were similarities and differences in their gene expressions and signaling pathways.Strengthening the body resistance of HQD is mainly related to adjusting Notch-induced EMT signaling pathway and eliminating pathogenic effects of YCHD is mostly regulated by cell adhesion-ECM pathway.