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Effects and modulating mechanism of iPSCs-MSCs on cholesterol concentration in macrophage foam cell
Author(s): LIANG Mei-ling, ZENG Wu-tao, CHEN Wei-yan, SUN Xiu-ting, YANG Yi-ying, SHI hui, LI Zheng-xun, Division of Cardiology, the First Affiliated Hospital of Sun Yat-sen University
Pages: 1010-
1013+1056
Year: 2015
Issue:
8
Journal: Journal of Tropical Medicine
Keyword: iPSCs-MSCs; foam cells; inflammation cytokine; ABCA1; Notch1;
Abstract: Objective This study is to investigate whether iPSCs-MSCs, mesenchymal stem cells derived from induced pluripotent stem cells influence the content of cholesterol in THP-1 macrophage foam cells and the probable modulating mechanism. Method The foam cell model and transwell model were established. After co-cultures of macrophage foam cell with iPSCs-MSCs, cellular cholesterol content was evaluated by cholesterol assay kit and cytokine levels were measured by an enzyme-linked immunosorbent assay. The expression of ABCA1(ATP-Binding Cassette Transporter A1)and Notch1 were detected by Western blot and Quantitative Real-time PCR. Results After co-cultures with iPSCsMSCs, cholesterol content in foam cell was reduced. Levels of TNF-α and IL-6 were decreased significantly. The expressions of m RNA and protein of ABCA1 were up-regulated(P <0.05). The expressions of m RNA and protein of Notch1 were down-regulated(P <0.05). Conclusions iPSCs-MSCs were capable of reducing cholesterol content of macrophage foam cell, which was probably related to up-regulation of ABCA1 expression and reduction of inflammation cytokines. Moreover, the function of iPSCs-MSCs that inhibited expression of inflammatory cytokines in foam cells might be associated with the inhibition of the Notch signaling pathway. This prompts iPSCs-MSCs may be applied to the prevention and treatment of atherosclerosis.
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