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Role of PCSK9 and IDOL in curcumin accelerating LDL-C uptake in HepG2 cells
Pages: 1286-1291
Year: Issue:  9
Journal: Chinese Pharmacological Bulletin

Keyword:  姜黄素低密度脂蛋白受体前蛋白转化酶枯草杆菌蛋白酶9型诱导低密度脂蛋白受体降解蛋白固醇调节元件结合蛋白23-羟基-3-甲基-戊二酰辅酶A还原酶;
Abstract: Aim To explore the lipid-lowering mecha-nisms of curcumin from the molecular levels and pro-vide scientific basis for clinical development of lipid-lowering drugs.Methods Using oil red O staining and enzymic to determinate the levels of cholesterol in HepG2 cells.Moreover,uptaking of DiI-LDL was also measured.The expressions of mRNA and protein were detected by RT-Q-PCR and Western blot.Results The red lipid droplets and the levels of TC and FC sig-nificantly increased in HepG2 cells after treated with curcumin.The orange red fluorescence was higher than that of control.Curcumin could promote the expression levels of mRNA and protein of SREBP2 and LDLR, what′s more,curcumin could reduce the expression of the mature PCSK9 level and IDOL protein.Conclu-sion Curcumin accelerates LDL-C uptake probably via downregulating the expression of PCSK9 and IDOL in HepG2 cells.
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