Subcutaneous Injection of Isoprenaline Induced Cardiac Hypertrophy in Mice
Author(s): ZHANG Wenbin, TANG Qizhu, LI Jin, XIANG Shizuo, HU Zhefu
Pages: 88-91
Year: 2015 Issue: 7
Journal: Journal of Medical Reaserch
Keyword: Isoprenaline; Animal model; Cardiac hypertrophy;
Abstract: Objective To investigate effects of subcutaneous injection of isoprenaline ( ISO) on cardiac hypertrophy in mice.Meth-ods Male C57 mice were divided into two groups:normal saline group ( NS group) and ISO group.The mice were subcutaneously injec-ted with a dose of 5mg/kg of ISO 2 times a day for two weeks, meanwhile mice in the NS group received the same volume of vehicle.The ejection fractions(EF%)were recorded after mice cardiac ultrasound.The heart weight(HW), lung weight(LW), and body weight(BW) were recorded and then the ratios of HW/BW and LW/BW were calculated.The cross-sectional areas ( CSA) of the myocytes was also counted.Atrial natriuretic peptide ( ANP) was detected by real time PCR.The signaling pathway involved in the cardiac hypertrophy was also detected by western blot.Results ISO induced reduced EF%,induced increased HW/BW, LW/BW, CSA of myocyte and mRNA level of ANP.And expressions of phospho-ERK and phospho-JNK in ISO group were enhanced compared with NS group.Conclusion Subcutaneous injections of ISO can effectively induce cardiac hypertrophy in mice model through the activation of MAPK signaling pathway.
Pages: 88-91
Year: 2015 Issue: 7
Journal: Journal of Medical Reaserch
Keyword: Isoprenaline; Animal model; Cardiac hypertrophy;
Abstract: Objective To investigate effects of subcutaneous injection of isoprenaline ( ISO) on cardiac hypertrophy in mice.Meth-ods Male C57 mice were divided into two groups:normal saline group ( NS group) and ISO group.The mice were subcutaneously injec-ted with a dose of 5mg/kg of ISO 2 times a day for two weeks, meanwhile mice in the NS group received the same volume of vehicle.The ejection fractions(EF%)were recorded after mice cardiac ultrasound.The heart weight(HW), lung weight(LW), and body weight(BW) were recorded and then the ratios of HW/BW and LW/BW were calculated.The cross-sectional areas ( CSA) of the myocytes was also counted.Atrial natriuretic peptide ( ANP) was detected by real time PCR.The signaling pathway involved in the cardiac hypertrophy was also detected by western blot.Results ISO induced reduced EF%,induced increased HW/BW, LW/BW, CSA of myocyte and mRNA level of ANP.And expressions of phospho-ERK and phospho-JNK in ISO group were enhanced compared with NS group.Conclusion Subcutaneous injections of ISO can effectively induce cardiac hypertrophy in mice model through the activation of MAPK signaling pathway.
Related Articles