The activation of SUMO-2/3 mediated protection effects of Salvia miltiorrhiza on ischemic neurons
Author(s): WANG Hui-gang, YU Si-miao, QIU Jin, LIU Zhen-lin, Xiaowangzhuang Community Health Service Center, Dagang District, Binhai New Area of Tianjin, Integrated Traditional Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Neurosurgenry Department, Tianjin Anjie Linao Brain Hospital
Pages: 420-423
Year: 2015 Issue: 7
Journal: Tianjin Journal of Traditional Chinese Medicine
Keyword: ischemic cerebrovascular disease; small ubiquitin like protein; Salvia miltiorrhiza; neuron;
Abstract: [Objective] To study the protection mechanism of salvia miltiorrhiza on neurons after ischemic cerebral vascular disease from the field of small ubiquitin-like protein(SUMOs). [Methods] The neurons were cultured from the hippocampal of SD rats, and identified by fluorescence immunoassay method. After making oxygen glucose deprivation(OGD) model for neurons, we designed three groups for this study: control group, OGD group and salvia miltiorrhiza group. The lactate dehydrogenase(LDH) content was detected by ELISA, and apoptosis by apoptosis in situ. Then the quantitative expression and intracellular localization of SUMO-1and SUMO-2/3 proteins were detected by western blot and immunofluorescence method. [Results] The cultured cells were with high expression of neuron specific enolase(NSE) and microtubule associated protein-2(MAP-2), but low level of glial fibrillary acidic protein(GFAP), the neuron purity greater than 95%. ELISA results showed that the content of LDH in the three groups were control group < Salvia miltiorrhiza group <OGD group, as well as the results from apoptosis. Western blotting results showed that the level of SUMO-2/3 of neurons received OGD treatment was raised significantly(P<0.05), but there was no significant change in SUMO-1(P>0.05), and salvia miltiorrhiza treatment can further improve the expression of SUMO-2/3(P<0.05). Immunohistochemistry results showed that there was apparent shift of SUMO-2/3 from cytoplasm to nucleus in OGD neurons, and salvia miltiorrhiza treatment could further enhance this effect. [Conclusion] Salvia miltiorrhiza can induce SUMO-2/3 activation and enhance protect effects on ischemic neurons.
Pages: 420-423
Year: 2015 Issue: 7
Journal: Tianjin Journal of Traditional Chinese Medicine
Keyword: ischemic cerebrovascular disease; small ubiquitin like protein; Salvia miltiorrhiza; neuron;
Abstract: [Objective] To study the protection mechanism of salvia miltiorrhiza on neurons after ischemic cerebral vascular disease from the field of small ubiquitin-like protein(SUMOs). [Methods] The neurons were cultured from the hippocampal of SD rats, and identified by fluorescence immunoassay method. After making oxygen glucose deprivation(OGD) model for neurons, we designed three groups for this study: control group, OGD group and salvia miltiorrhiza group. The lactate dehydrogenase(LDH) content was detected by ELISA, and apoptosis by apoptosis in situ. Then the quantitative expression and intracellular localization of SUMO-1and SUMO-2/3 proteins were detected by western blot and immunofluorescence method. [Results] The cultured cells were with high expression of neuron specific enolase(NSE) and microtubule associated protein-2(MAP-2), but low level of glial fibrillary acidic protein(GFAP), the neuron purity greater than 95%. ELISA results showed that the content of LDH in the three groups were control group < Salvia miltiorrhiza group <OGD group, as well as the results from apoptosis. Western blotting results showed that the level of SUMO-2/3 of neurons received OGD treatment was raised significantly(P<0.05), but there was no significant change in SUMO-1(P>0.05), and salvia miltiorrhiza treatment can further improve the expression of SUMO-2/3(P<0.05). Immunohistochemistry results showed that there was apparent shift of SUMO-2/3 from cytoplasm to nucleus in OGD neurons, and salvia miltiorrhiza treatment could further enhance this effect. [Conclusion] Salvia miltiorrhiza can induce SUMO-2/3 activation and enhance protect effects on ischemic neurons.
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