Effects of Bingxiang Powder on Lymphocyte Proliferation and Cytokine Secretion in Mice Infected with Influenza A
Author(s): XIE Wenfang, DING Wei, DAI Wanjuan, JIANG Yi, XU Xiuni, SHAO Minming, LIU Ni, ZHAO Fang, ZHANG Fengxue, XU Peiping, WANG Xinhua, Institute of Tropical Medicine of Guangzhou University of Chinese Medicine, Guangzhou Medical University
Pages: 578-581
Year: 2013 Issue: 6
Journal: Traditional Chinese Drug Research & Clinical Pharmacology
Keyword: Bingxiang Powder; Different administration methods; Influenza A; Lymphocyte proliferation; Cytokines;
Abstract: Objective To observe the effect of volatile oil of Bingxiang Powder on lymphocyte proliferation and cytokine secretion in mice infected with influenza A(H1N1)virus by different administration methods,and to explore its possible mechanism during immune response.Methods We administered the essential oil of Bingxiang Powder by dripping nose or by atomization,and then used CCK-8 to detect the proliferation of lymphocyte T and B induced by concanavalin(ConA)and lipopolysaccharide(LPS),and used enzyme linked immunosorbent assay(ELISA)to detect the levels of interleukin 4(IL-4),interleukin 2(IL-2),interferon gamma(IFN-γ)and tumor necrosis factor alpha(TNF-α).Results H1N1 showed obvious inhibitory effect on the proliferation of mice splenic lymphocyte B(P < 0.05 compared with that in the normal control group),increased the levels of inflammatory factors of TNF-α,IFN-γ and IL-2(P < 0.05),but had no effect on IL-4 level.Ribovirin enhanced the splenic lymphocyte B proliferation(P < 0.01),increased IL-4 and IFN-γ levels and decreased TNF-α leve(lP < 0.05).Bingxiang Powder by dripping nose or by atomization promoted splenic lymphocyte B and T proliferation,and inhibited the secretion of IL-2 in mice infected with H1N1(P < 0.05).The administration of Bingxiang Powder by dripping nose could also inhibit the secretion of TNF-α(P < 0.05),and Bingxiang Powder by atomization promoted production of IL-4 and IFN-γ(P < 0.05).Conclusion The volatile oil of Bingxiang Powder has certain effect on promoting the activates of spenic lymphocytes in mice infected with influenza A,and can regulate the balance of Th1/Th2.The anti-viral immunoregulation mechanism varies in the administration methods of dripping nose or atomization.
Pages: 578-581
Year: 2013 Issue: 6
Journal: Traditional Chinese Drug Research & Clinical Pharmacology
Keyword: Bingxiang Powder; Different administration methods; Influenza A; Lymphocyte proliferation; Cytokines;
Abstract: Objective To observe the effect of volatile oil of Bingxiang Powder on lymphocyte proliferation and cytokine secretion in mice infected with influenza A(H1N1)virus by different administration methods,and to explore its possible mechanism during immune response.Methods We administered the essential oil of Bingxiang Powder by dripping nose or by atomization,and then used CCK-8 to detect the proliferation of lymphocyte T and B induced by concanavalin(ConA)and lipopolysaccharide(LPS),and used enzyme linked immunosorbent assay(ELISA)to detect the levels of interleukin 4(IL-4),interleukin 2(IL-2),interferon gamma(IFN-γ)and tumor necrosis factor alpha(TNF-α).Results H1N1 showed obvious inhibitory effect on the proliferation of mice splenic lymphocyte B(P < 0.05 compared with that in the normal control group),increased the levels of inflammatory factors of TNF-α,IFN-γ and IL-2(P < 0.05),but had no effect on IL-4 level.Ribovirin enhanced the splenic lymphocyte B proliferation(P < 0.01),increased IL-4 and IFN-γ levels and decreased TNF-α leve(lP < 0.05).Bingxiang Powder by dripping nose or by atomization promoted splenic lymphocyte B and T proliferation,and inhibited the secretion of IL-2 in mice infected with H1N1(P < 0.05).The administration of Bingxiang Powder by dripping nose could also inhibit the secretion of TNF-α(P < 0.05),and Bingxiang Powder by atomization promoted production of IL-4 and IFN-γ(P < 0.05).Conclusion The volatile oil of Bingxiang Powder has certain effect on promoting the activates of spenic lymphocytes in mice infected with influenza A,and can regulate the balance of Th1/Th2.The anti-viral immunoregulation mechanism varies in the administration methods of dripping nose or atomization.
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