Author(s): Yan Si-zhuang, Wang Qi-nan, Du Ji-zhao and Zheng Xing-pin
Pages: 267-271
Year: 1989 Issue:  4
Journal: Chinese Journal of Antibiotics
Keyword:  Ceftriaxone; High and Sub-inhibitory Concentration; Bacteria; Ultra-Structural Changes;
Abstract: The morphlogical and ultra-structural changes of three strains of S. aureus, E, coli and P. aeruginosa were observed under electron microscope after exposure to both high and Sub-inhibitory concentrations of ceftriaxone for three and eighteen hours, respectively. The results showed that the structural changes were almost identical between the bacteria exposing for three hours and the ones for eighteen hours. Shrinking of the cell membrane, rupture of cell wall or even Sysis of the cell were seen when the bacteria were effected by ceftriaxone in the concentration equal to or more ’han 10MIC. When grew with Sub-MIC of ceftriaxone, the S. aureus became larger with irregular shape, decreased dense and a thick cross wall, and the E. coli and P. aeruginosa presented elongation or even filamentation with or without vacuoles in the cell. Our results confirmed previous findings and indicated that ceftriaxone is a bactericidal antibiotic and mainly binds PBP3 of E. coli and P. aeruginosa.