RATIONAL SCREENING OF THE SPIRAMYCIN-PRODUCING STRAIN STREPTOMYCES SPIRAMYCETICUS RESISTING TO CARBON CATABOLITE REPRE-SSIONIRG
Author(s): Luo Jing-qing Li You-rong
Pages: 243-246
Year: 1989 Issue: 4
Journal: Chinese Journal of Antibiotics
Keyword: Rational screening; 2-deoxy-D-glucose; Spriamycin; U.V. mutation;
Abstract: Spiramycin produced by Streptomyces spiramyceticus A. sp. 799 is Subject to glucose catabolite repression. After the suspension of spores was treated by UV, we isolated the resistant strains which could grow on the medium contain ing 2 -deoxy-D-glucose. The mutaats obtained by above method produced much more spiram-ycin than those obtained by random screening after UV treatment. The productivity of mutants can be increased 78. 9 % in a certain concentration of 2 -deoxy-D-gluco-se.
Pages: 243-246
Year: 1989 Issue: 4
Journal: Chinese Journal of Antibiotics
Keyword: Rational screening; 2-deoxy-D-glucose; Spriamycin; U.V. mutation;
Abstract: Spiramycin produced by Streptomyces spiramyceticus A. sp. 799 is Subject to glucose catabolite repression. After the suspension of spores was treated by UV, we isolated the resistant strains which could grow on the medium contain ing 2 -deoxy-D-glucose. The mutaats obtained by above method produced much more spiram-ycin than those obtained by random screening after UV treatment. The productivity of mutants can be increased 78. 9 % in a certain concentration of 2 -deoxy-D-gluco-se.
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