INHIBITORY EFFECT OF ACTINOMYCIN 23-21 ON HELA CELL LINE
Author(s): Li Chang-chuin and Li Tong-guan Wu De-feng, Zhang Yi, Zheng Zhi-hong and Fang Ce Liang Ping
Pages: 421-424
Year: 1988 Issue: 6
Journal: Chinese Journal of Antibiotics
Keyword: Actinomycin 23-21; HeLa cell;
Abstract: The present study deals with a novel anticancer antibiotic,actinomycin 23-21,isolated from Fuzhou soil.HeLa cells were cultured in the medium consisting of RPM1 1640 and bovine serum,and treated with different concentrations of the drug. According to the dose-response curve,the concentration corresponding to 50% survival rate was taken for experimental use,which contained actinomycin 23-21 20μg/ml. The growth curve of HeLa cells treated with the drug and their growing ability on soft agar all indicated that actinomycin 23-21 could inhibit the growth of HeLa cells. Reproduction and colony formation of HeLa cells were also inhibited by the drug. Some structural changes such as swelling and breaking-up of HeLa cells treated with the drug were observed by microscopy. Clumping of peripheral heterochromatin,shrinkage of nucleolus,and separation of nucleolus with nucleo-associated chromatin could be seen under Electron microscope(×10,000). Immunosuppressed mice inoculated with HeLa cells treated with actinomycin 23-21 did not develop neoplasms. Meanwhile,epithelioma developed in the mice of control group. All of these results confirmed the inhibitory effect of actinomyciu 23-21 on HeLa cell line.
Pages: 421-424
Year: 1988 Issue: 6
Journal: Chinese Journal of Antibiotics
Keyword: Actinomycin 23-21; HeLa cell;
Abstract: The present study deals with a novel anticancer antibiotic,actinomycin 23-21,isolated from Fuzhou soil.HeLa cells were cultured in the medium consisting of RPM1 1640 and bovine serum,and treated with different concentrations of the drug. According to the dose-response curve,the concentration corresponding to 50% survival rate was taken for experimental use,which contained actinomycin 23-21 20μg/ml. The growth curve of HeLa cells treated with the drug and their growing ability on soft agar all indicated that actinomycin 23-21 could inhibit the growth of HeLa cells. Reproduction and colony formation of HeLa cells were also inhibited by the drug. Some structural changes such as swelling and breaking-up of HeLa cells treated with the drug were observed by microscopy. Clumping of peripheral heterochromatin,shrinkage of nucleolus,and separation of nucleolus with nucleo-associated chromatin could be seen under Electron microscope(×10,000). Immunosuppressed mice inoculated with HeLa cells treated with actinomycin 23-21 did not develop neoplasms. Meanwhile,epithelioma developed in the mice of control group. All of these results confirmed the inhibitory effect of actinomyciu 23-21 on HeLa cell line.
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