SEPARATION,IDENTIFICATION AND RENAMING OF THE MAIN COMPOSITION OF CHINESE NYSTATIN
Author(s): Ma Jian-wen, Liu Yu-bo, Wen De-xiu and Zhu Feng-juan Wang Shao-wen and Ling Da-kui
Pages: 83-90
Year: 1987 Issue: 2
Journal: Chinese Journal of Antibiotics
Keyword: 制霉素; 快原子轰击质谱; 分离制备; 旋光度; 核磁共振谱; 英国药典; 荧光光谱; 碎片峰; 测试数据; 旋转薄膜蒸发器;
Abstract: In previous paper[5], we reported that the main components C1, C2 were contained in Chinese nystatin but nystatin A, was the main component of nystatin outsido China.The purified samples C1, and C2（from Chinese working standard for nystatin） and nystatin A1,（from American product） were prepared by HPLC using a semi-preparative column（0.7× 25cm,10μm Nucleosil ODS）.C1 and C2 were identified to be nystatin A3, and polyfungin B respectively by using UV, IR, Fluorescence spectrum, specific rotatory,1HNMR, FABMS, The potencies were determined according to the Ch. P. （1977） resulted as Follows, C1, 3217 μ/mg±4.4%, C2 10879μ/mg±4.4%, nystatin A1, 3956 ±5.5%. Therefore, in order to distinguish Chinese nystatin from nystatin outside China, we suggested that the new name Chinyfungin replaced the present Chinese nystatin.
Pages: 83-90
Year: 1987 Issue: 2
Journal: Chinese Journal of Antibiotics
Keyword: 制霉素; 快原子轰击质谱; 分离制备; 旋光度; 核磁共振谱; 英国药典; 荧光光谱; 碎片峰; 测试数据; 旋转薄膜蒸发器;
Abstract: In previous paper[5], we reported that the main components C1, C2 were contained in Chinese nystatin but nystatin A, was the main component of nystatin outsido China.The purified samples C1, and C2（from Chinese working standard for nystatin） and nystatin A1,（from American product） were prepared by HPLC using a semi-preparative column（0.7× 25cm,10μm Nucleosil ODS）.C1 and C2 were identified to be nystatin A3, and polyfungin B respectively by using UV, IR, Fluorescence spectrum, specific rotatory,1HNMR, FABMS, The potencies were determined according to the Ch. P. （1977） resulted as Follows, C1, 3217 μ/mg±4.4%, C2 10879μ/mg±4.4%, nystatin A1, 3956 ±5.5%. Therefore, in order to distinguish Chinese nystatin from nystatin outside China, we suggested that the new name Chinyfungin replaced the present Chinese nystatin.
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