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China/Asia On Demand
Journal Articles
Pages: 276-280
Year: Issue:  5
Journal: Chinese Journal of Antibiotics

Keyword:  氨噻肟头孢菌素超微结构抗生素耐药菌抗菌活性第三代头孢菌素临床前药效学金黄色葡萄球菌最低抑菌浓度金色葡萄球菌;
Abstract: In vitro the MICs of Cefotaxime and Penicillin G to Staphytococcus aureus were very similar from 0.01 to 0.05 μg/ml. To E. coli the former was more active than the latter, The MIC was<0.01 and l2.5μg/ml respectively.The ultrastructural changes ware examined by electron microscopy. After three hours exposure of susceptible Staphylococci fo Penicillin G with the concentrations of 1 and 50 μg/ml, the septa showed broader and loss in density, irregular in shape, and the Peripheral cell wall ware markedly thin. Occaoionally the extruding cytoplasm and the clearly broken cell walls were observed.In higher concentration, some membrane-like rings were present. The home made Cefotaxime and Cefotaxime made in Germany in concentration of 20μg/ml, after 1 or 3 hours exposure,the ultrastructural changes of S. aureus were similar to Penicillin G,the lysis was apparent, cell wall defects and fragments generally observed.E.coli,after 1 or 3 hours exposure to 1 or 50μg/ml Penicillin G showed no apparent morphological changes examined by electron microscopy.Nevertheless, When the Cefotaxime concentration were 2 or 20 μg/ml, after 1 or 3 hours exposure, a dramatic ultrastructural changes of E.coli were observed. The cell walls were rough, blurring or irregular in shape. Numerous membrane-like irregular in shaped rings and debris were generally seen. A classical lysis and single or twin bulges very similar to those seen when E. coli was exposed to MT-141 or Colicin were observed.These findings suggest that Cefotaxime is a bactericidal drug to S. aureus and E. coli in resulting the cell wall defects and cell lysis.
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