THE ULTRASTRUCTURAL CHANGES OF MICRO-ORGANISMS AFTER EXPOSURE TO CEFOTAXIME
Author(s): Liang Yong-yu, Wang Yu-sheng, Zhao Shu-qin and Li Guang-rong
Pages: 276-280
Year: 1985 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 氨噻肟头孢菌素; 超微结构; 抗生素耐药菌; 抗菌活性; 第三代头孢菌素; 临床前; 药效学; 金黄色葡萄球菌; 最低抑菌浓度; 金色葡萄球菌;
Abstract: In vitro the MICs of Cefotaxime and Penicillin G to Staphytococcus aureus were very similar from 0.01 to 0.05 μg/ml. To E. coli the former was more active than the latter, The MIC was<0.01 and l2.5μg/ml respectively.The ultrastructural changes ware examined by electron microscopy. After three hours exposure of susceptible Staphylococci fo Penicillin G with the concentrations of 1 and 50 μg/ml, the septa showed broader and loss in density, irregular in shape, and the Peripheral cell wall ware markedly thin. Occaoionally the extruding cytoplasm and the clearly broken cell walls were observed.In higher concentration, some membrane-like rings were present. The home made Cefotaxime and Cefotaxime made in Germany in concentration of 20μg/ml, after 1 or 3 hours exposure,the ultrastructural changes of S. aureus were similar to Penicillin G,the lysis was apparent, cell wall defects and fragments generally observed.E.coli,after 1 or 3 hours exposure to 1 or 50μg/ml Penicillin G showed no apparent morphological changes examined by electron microscopy.Nevertheless, When the Cefotaxime concentration were 2 or 20 μg/ml, after 1 or 3 hours exposure, a dramatic ultrastructural changes of E.coli were observed. The cell walls were rough, blurring or irregular in shape. Numerous membrane-like irregular in shaped rings and debris were generally seen. A classical lysis and single or twin bulges very similar to those seen when E. coli was exposed to MT-141 or Colicin were observed.These findings suggest that Cefotaxime is a bactericidal drug to S. aureus and E. coli in resulting the cell wall defects and cell lysis.
Pages: 276-280
Year: 1985 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 氨噻肟头孢菌素; 超微结构; 抗生素耐药菌; 抗菌活性; 第三代头孢菌素; 临床前; 药效学; 金黄色葡萄球菌; 最低抑菌浓度; 金色葡萄球菌;
Abstract: In vitro the MICs of Cefotaxime and Penicillin G to Staphytococcus aureus were very similar from 0.01 to 0.05 μg/ml. To E. coli the former was more active than the latter, The MIC was<0.01 and l2.5μg/ml respectively.The ultrastructural changes ware examined by electron microscopy. After three hours exposure of susceptible Staphylococci fo Penicillin G with the concentrations of 1 and 50 μg/ml, the septa showed broader and loss in density, irregular in shape, and the Peripheral cell wall ware markedly thin. Occaoionally the extruding cytoplasm and the clearly broken cell walls were observed.In higher concentration, some membrane-like rings were present. The home made Cefotaxime and Cefotaxime made in Germany in concentration of 20μg/ml, after 1 or 3 hours exposure,the ultrastructural changes of S. aureus were similar to Penicillin G,the lysis was apparent, cell wall defects and fragments generally observed.E.coli,after 1 or 3 hours exposure to 1 or 50μg/ml Penicillin G showed no apparent morphological changes examined by electron microscopy.Nevertheless, When the Cefotaxime concentration were 2 or 20 μg/ml, after 1 or 3 hours exposure, a dramatic ultrastructural changes of E.coli were observed. The cell walls were rough, blurring or irregular in shape. Numerous membrane-like irregular in shaped rings and debris were generally seen. A classical lysis and single or twin bulges very similar to those seen when E. coli was exposed to MT-141 or Colicin were observed.These findings suggest that Cefotaxime is a bactericidal drug to S. aureus and E. coli in resulting the cell wall defects and cell lysis.
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