Author(s): Wu Zhang-xiu Li Jun-ying and Cai Shun-yang Zhang Xiao-yu
Pages: 316-322
Year: 1984 Issue:  4
Journal: Chinese Journal of Antibiotics
Keyword:  分光光度法测定; 发酵液; 吸光值; 醋酸镉; 薄层层析分离; 分光光度测定法; 硅胶板; 茚三酮; 含量测定; 醋酸钠;
Abstract: A simple, selective and accurate spect-rophotometric method for individual determination of Kanamycin A and B in culture broth is described. These components can be separated by thin layer chromatographyon silica gel H plates. Ninhydria-cadmium acetate reagent system is used for monitoring the color-producing reaction. The separation and detection procedure are easy to perform.