STUDIES ON ENZYME INHIBITOR PRODUCED BY MICROORGANISM—— Ⅱ. PRELIMINARY REPORT ON THE PRODUCTION OF THE CHYMOTRYPSIN INHIBITOR PRODUCED BY STREPTOMYCES S-81-24
Author(s): Liu Hua-zhen, Chen Bi-e Wang Yue Liu Qi-ying
Pages: 280-284
Year: 1983 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 糜蛋白酶抑制剂; S-81-24; 链霉菌; 非极性有机溶剂; 大孔树脂吸附; 抑制作用; 摇瓶培养; 紫外吸收峰; 多功能性; 乙酸乙醋;
Abstract: The production of the chymotrypsin inhibitor by Streptomyces S-81-24 reached a maximum after two to three days in submerged culture. Chymotrypsin inhibitor can be extracted either by absorption using D-resin or with n-butanol at pH 2~3. It is soluble in water and methanol, but insoluble in non-polar solvents, such as, ethyl-acetate, ether, benzene, chloroform etc. Novisible absorbtion is observed in U. V. spectrum. It inhibits not only chymotrypsin strongly but also inhibits trypsin and pepsin to a certain extent. Whether this poly-functional action is due to the inhibitor as a whole or due to the different components of the substance needs further investiga-tion.
Pages: 280-284
Year: 1983 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 糜蛋白酶抑制剂; S-81-24; 链霉菌; 非极性有机溶剂; 大孔树脂吸附; 抑制作用; 摇瓶培养; 紫外吸收峰; 多功能性; 乙酸乙醋;
Abstract: The production of the chymotrypsin inhibitor by Streptomyces S-81-24 reached a maximum after two to three days in submerged culture. Chymotrypsin inhibitor can be extracted either by absorption using D-resin or with n-butanol at pH 2~3. It is soluble in water and methanol, but insoluble in non-polar solvents, such as, ethyl-acetate, ether, benzene, chloroform etc. Novisible absorbtion is observed in U. V. spectrum. It inhibits not only chymotrypsin strongly but also inhibits trypsin and pepsin to a certain extent. Whether this poly-functional action is due to the inhibitor as a whole or due to the different components of the substance needs further investiga-tion.
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