STUDIES ON THE MODE ACTION OF A CHINA MADE ANTIBIOTIC RIFANDIN (R76-1) ON BACTERIAL MACROMOLECULAR SYNTHESIS
Author(s): Zhu Cai-rong Wang Yu-sheng
Pages: 295-301
Year: 1983 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 力复霉素; 抗菌作用; 半合成衍生物; 掺入法; 最低杀菌浓度; 金色葡萄球菌; 甲苯闪烁液; 抑制作用; 二倍稀释法; 药对;
Abstract: This paper reported the preliminary study of the mode of action of a new China made Rifamycin derivative-Rifandin(R-76-l) on bacterial macromolecular synthesis. The antibacterial action of R-76-1 to Staphylo-coccus aureus and E. coli was very similar to Rifampin determined by double dilution tests. The radio labelled precursors incorporation experiment showed that R-76-1 inhibited RNA synthesis rapidly similar as Rifampin, but no significant effect to DNA and protein synthesis of the bacteria. The degree of inhibition of R-76-1 and Rifampin to RNA synthesis of E. coli #2281 were linearly related to the concentration of the drugs. These results suggested that both of them affected the DNA dependent RNA po-lymerase activity of the microorganism.Chemical quantitative assay of RNA and protein content in E. coli strain 314 and 315 in vitro after treated with R-76-1 and Rifampin demonstrated similar results as the incorporation studies.Test bacteria pretreated with 0.08 mM EDTA showed at the same concentration the inhibition of RNA synthesis by Rifandin was significantly stronger than Rifampin.So it is reasonable to consider that R-76-1 had a greater affinity and potency of inhibition to RNA polymerase than Rifampin. Even though the precise mechanism of R-76-1 to RMA polymerase remained for further investigation.
Pages: 295-301
Year: 1983 Issue: 5
Journal: Chinese Journal of Antibiotics
Keyword: 力复霉素; 抗菌作用; 半合成衍生物; 掺入法; 最低杀菌浓度; 金色葡萄球菌; 甲苯闪烁液; 抑制作用; 二倍稀释法; 药对;
Abstract: This paper reported the preliminary study of the mode of action of a new China made Rifamycin derivative-Rifandin(R-76-l) on bacterial macromolecular synthesis. The antibacterial action of R-76-1 to Staphylo-coccus aureus and E. coli was very similar to Rifampin determined by double dilution tests. The radio labelled precursors incorporation experiment showed that R-76-1 inhibited RNA synthesis rapidly similar as Rifampin, but no significant effect to DNA and protein synthesis of the bacteria. The degree of inhibition of R-76-1 and Rifampin to RNA synthesis of E. coli #2281 were linearly related to the concentration of the drugs. These results suggested that both of them affected the DNA dependent RNA po-lymerase activity of the microorganism.Chemical quantitative assay of RNA and protein content in E. coli strain 314 and 315 in vitro after treated with R-76-1 and Rifampin demonstrated similar results as the incorporation studies.Test bacteria pretreated with 0.08 mM EDTA showed at the same concentration the inhibition of RNA synthesis by Rifandin was significantly stronger than Rifampin.So it is reasonable to consider that R-76-1 had a greater affinity and potency of inhibition to RNA polymerase than Rifampin. Even though the precise mechanism of R-76-1 to RMA polymerase remained for further investigation.
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