Author(s): HU Ling-ying, ZHANG Nian-zhang, GAO Qi, ZHU Xing-quan, WANG Shou-kun, Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, South China Agricultural University
Pages: 270-274
Year: 2015 Issue:  2
Journal: China Animal Husbandry & Veterinary Medicine
Keyword:  Toxoplasma gondii; Toxoplasma gondii nuclear factor 3(TgNF3)gene; clone; bioin-formatics analysis;
Abstract: In order to predict the possibility of Toxoplasma gondii nuclear factor 3(TgNF3)as anti-Toxoplasma gondii vaccine candidate,TgNF3 gene was amplified by one step RT-PCR using apair of specific primers.Then the fragments were cloned into the pMD18-T vector.The sequence of TgNF3 gene was then translated into amino acids and analyzed by bioinformatics software.The physical and chemical characteristics,hydrophilic domain,transmembrane domain,epitopes,and advanced structure of TgNF3 were predicted by multiple bioinformatics approaches.The results revealed that TgNF3 gene was approximate 950 bp in length.TgNF3 protein was predicted to contain only one transmembrane domain,9α-helix regions,4β-fold regions,8hydrophilic regions and 10 flexible regions,and predicted to contain 9linear B cell epitopes.The results indicated that TgNF3 could be a vaccine candidate antigen against Toxoplasma gondii,which provided the basic data for development of novel Toxoplasma gondii vaccines.