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Author:
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Issue:
tong guo dna zhong zu ji shu sheng chan yi miao
Author(s):
LEI Guanyu
,
j.s.emtage
,
n.h.carey
Pages:
48
-
50
Year:
1983
Issue:
6
Journal:
China Animal Husbandry & Veterinary Medicine
Keyword:
DNA重组技术
;
生产疫苗
;
粘性末端
;
基因克隆
;
真核生物
;
重组体
;
分子克隆
;
克隆基因
;
操纵基因
;
细胞总蛋白
;
Abstract:
<正> 前言我们今天所知道的 DNA 重组技术是把种种观察、发现和推论融合起来而产生的。首先,限制性核酸内切酶的发现(Smith,1979)使能在特定位点切开 DNA,有时造成重叠的粘性末端。因而,利用 DNA 连接酶能把相同或
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