Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats
Author(s): YANG Yi, ZHANG Ji-kun, XIN Hai-liang, TIAN Xing-zhou, HAN Xiao-jie, DUAN Yong-bang, WANG Dai-lin, WU Wen-xuan, LI Sheng-li, College of Animal Science, Guizhou University, Kaili Bureau of Animal Husbandry, Key Laboratory of Animal Nutrition
Pages: 932-942
Year: 2014 Issue: 6
Journal: Chinese Journal of Animal and Veterinary Sciences
Keyword: dietary cation-anion difference; blood calcium; calcium binding protein-9kDa; transition goats;
Abstract: The study was conducted to clarify the mechanism of low dietary cation-anion difference(DCAD)level preventing hypocalcemia in transition cows by investigating the influence of DCAD levels on blood Ca concentration and calcium binding protein-9kDa(CaBP-D9k)expression level in gastrointestinal tract of transition goats.Twenty-seven Guizhou Black goats in transition period were randomly allocated to 3treatments with 9goats each treatment and were fed 3diets with varying DCAD levels:high DCAD(HD,+325),control(CON,+179)and low DCAD(LD,-110)groups,respectively.Goats were blooded on d 10before lambing,d 0for lambing and d 3afterlambing,respectively,and were slaughtered to detect the plasma parameters and CaBP-D9kexpression level of gastrointestinal tract.Compared with high DCAD,feed intake was unaffected by low DCAD treatment(P>0.05),urine pH of goats fed LD was reduced with decreasing DCAD level(P<0.05).Compared to CON and HD,reducing DCAD level induced higher plasma Cl-and Ca2+concentration of goats during the experiment(P<0.05).Greater CaBP-D9kmRNA expression level was observed in whole gastrointestinal tract of LD-fed goats except for ileum,cecum and rectum compared with goats in HD group(P<0.05).Moreover,feeding LD diet relieved the decline of CaBP-D9kmRNA expression level in abomasum,jejunum and colon after lambing(P<0.05).In conclusion,reducing DCAD could up-regulate CaBP-D9kexpression level in stomach,proximal intestine and colon,and kept Ca2+concentration for transition goats.The results indicated that Ca2+absorption in the above gastrointestinal tracts was increased and would be the possible mechanism of low DCAD enhancing blood Ca homeostasis in dairy cows during periparturient period.
Pages: 932-942
Year: 2014 Issue: 6
Journal: Chinese Journal of Animal and Veterinary Sciences
Keyword: dietary cation-anion difference; blood calcium; calcium binding protein-9kDa; transition goats;
Abstract: The study was conducted to clarify the mechanism of low dietary cation-anion difference(DCAD)level preventing hypocalcemia in transition cows by investigating the influence of DCAD levels on blood Ca concentration and calcium binding protein-9kDa(CaBP-D9k)expression level in gastrointestinal tract of transition goats.Twenty-seven Guizhou Black goats in transition period were randomly allocated to 3treatments with 9goats each treatment and were fed 3diets with varying DCAD levels:high DCAD(HD,+325),control(CON,+179)and low DCAD(LD,-110)groups,respectively.Goats were blooded on d 10before lambing,d 0for lambing and d 3afterlambing,respectively,and were slaughtered to detect the plasma parameters and CaBP-D9kexpression level of gastrointestinal tract.Compared with high DCAD,feed intake was unaffected by low DCAD treatment(P>0.05),urine pH of goats fed LD was reduced with decreasing DCAD level(P<0.05).Compared to CON and HD,reducing DCAD level induced higher plasma Cl-and Ca2+concentration of goats during the experiment(P<0.05).Greater CaBP-D9kmRNA expression level was observed in whole gastrointestinal tract of LD-fed goats except for ileum,cecum and rectum compared with goats in HD group(P<0.05).Moreover,feeding LD diet relieved the decline of CaBP-D9kmRNA expression level in abomasum,jejunum and colon after lambing(P<0.05).In conclusion,reducing DCAD could up-regulate CaBP-D9kexpression level in stomach,proximal intestine and colon,and kept Ca2+concentration for transition goats.The results indicated that Ca2+absorption in the above gastrointestinal tracts was increased and would be the possible mechanism of low DCAD enhancing blood Ca homeostasis in dairy cows during periparturient period.
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