Protective Effect of Tannins from Sanguisorba officinalis on Cyclophosphamide Induced Myelosuppression in Mice
Author(s): XIONG Yong-ai, ZOU Jun-bo, YANG Min, Pharmaceutical College, Chengdu University of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Key Lab. of Modern Preparation of Traditional Chinese Medicine, Ministry of Education
Pages: 499-503
Year: 2014 Issue: 4
Journal: Natural Product Research and Development
Keyword: Great Burnet tannins; cyclophosphamide; myelosuppression; antagonism;
Abstract: The objective of this study was to investigate the protective effect of tannins from Sanguisorba officinalis( Great Burnet) on myelosuppression in mice. The myelosuppression model was induced by peritoneal injection of cyclophosphamide( CTX). Then the myelosuppression mice were randomly divided into: model group,colony-stimulating factor group,three Great Burnet tannins treatment groups of different dosage( 20,10,5 mg / kg),and normal group. The drugs were chronically administrated to myelosuppression mice once a day for 21 days. The counts of peripheral blood leukocytes,DNA contents and CD34+expression of bone marrow cells and the O6-methyl guanine-DNA methyltransferase( MGMT) protein expression were used as main indexes and were determined. The results showed that Great Burnet tannins significantly increased peripheral WBC( P < 0. 05); significantly elevated bone marrow DNA content( P < 0. 05); significantly promoted the expression of CD34+( P < 0. 05); also significantly promoted MGMT protein expression( P < 0. 05). Based on these results,it was concluded that Great Burnet tannins can significantly antagonize CTX induced myelosuppression in mice.
Pages: 499-503
Year: 2014 Issue: 4
Journal: Natural Product Research and Development
Keyword: Great Burnet tannins; cyclophosphamide; myelosuppression; antagonism;
Abstract: The objective of this study was to investigate the protective effect of tannins from Sanguisorba officinalis( Great Burnet) on myelosuppression in mice. The myelosuppression model was induced by peritoneal injection of cyclophosphamide( CTX). Then the myelosuppression mice were randomly divided into: model group,colony-stimulating factor group,three Great Burnet tannins treatment groups of different dosage( 20,10,5 mg / kg),and normal group. The drugs were chronically administrated to myelosuppression mice once a day for 21 days. The counts of peripheral blood leukocytes,DNA contents and CD34+expression of bone marrow cells and the O6-methyl guanine-DNA methyltransferase( MGMT) protein expression were used as main indexes and were determined. The results showed that Great Burnet tannins significantly increased peripheral WBC( P < 0. 05); significantly elevated bone marrow DNA content( P < 0. 05); significantly promoted the expression of CD34+( P < 0. 05); also significantly promoted MGMT protein expression( P < 0. 05). Based on these results,it was concluded that Great Burnet tannins can significantly antagonize CTX induced myelosuppression in mice.
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