Author(s): LIU Gui-ying, ZHANG Hui-qi, YIN Xin-zhong, ZHU Zhen-gang, LIU Min, QIAO Ya-zhen, DOU Ying-ting, LIU Wen-bin, LIU Chao-wu, DI Guan-lin, CHANG Li, TIAN Chang-hong
Pages: 159-163
Year: 2013 Issue:  3
Journal: Tianjin Journal of Traditional Chinese Medicine
Keyword:  asthma; eosinophils; transmembrane migration; Shufeng Tongluo prescription; gene expression;
Abstract: [Objective] To explore the effect of Shufeng Tongluo decoction on eosinophils transendothelia migration in lung of rats with asthma.[Methods] Seventy Wistar rats(SPF) were randomly divided into seven groups: normal group,model group,dexamethasone group,DPI group,low-dose group of Chinese medicine,medium-dose group of Chinese medicine,and high-dose group of Chinese medicine with 10 rats in each group.The rats in normal group were sensitized by 1 mL of intra-peritoneal injection of normal saline.The other 6 groups were given with 1 mL sensitization liquid,contained 100 mg OVA(Grade V),100 mg aluminum hydroxide and 6×109 inactivated whooping cough bacili on the first day of experimental study.From 15th day,the rats in normal group were administrated with 1 mL of normal saline orally,2 hours later the rats were challenged by 6 mL normal saline in a box of aerosol inhalation once a day for 30 min a time and keeping for 10 days;the rats in other 6 group were respectively administrated with corresponding drugs orally and 2 hours later were challenged by 6 mL OVA(5%,V Grade) of aerosol inhalation once a day,for 30 min a time and keeping for 10 days,And 10 days later all rats were killed.The samples from rats were collected for examination.[Results] The expression of VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2 related factors to eosinophils transendothelia migration in lung of rat with asthma were significantly higher than that of normal group rats(P<0.001).Compared with the model group,dexamethasone group could significantly inhibit the expression of above factors(P<0.001).The effect of high-dose group of Chinese medicine was similar to dexamethasone group(P>0.05),the other treatment groups(DPI group,low-dose group of Chinese medicine and medium-dose group of Chinese medicine) only had general inhibition effect.[Conclusion] Shufeng Tongluo decoction has some certain suppression effect on eosinophils transendothelia migration in lung of rats with asthma to a certain extent.It may be one of its action mechanisms in controlling asthma.