Extraction and structural identification of β-1,3-D-glucan from yeast
Author(s): FENG Wen-juan, XU Ze-ping, ZHOU Chuan-bing, HAN Xiao-yang, LIU Jie-lei, Yellow River Delta JingBo Research Institute of Chemical Industry Co., Ltd
Pages: 151-153
Year: 2013 Issue: 5
Journal: Food & Machinery
Keyword: yeast cell wall; β-1; 3-D-glucan; alkali-enzyme method; structure;
Abstract: Studied the method of extraction and separation of β-1,3-Dglucan from yeast and identified its structure.The yeast polysaccharide was extracted by alkali-enzyme method,then,the structure of yeast glucan was analyzed by IR,and its monosaccharide compositions were analyzed by HPLC.Two fractions of polysaccharides were isolated and identified as YPⅠand YP,Ⅱrespectively,by alkali-enzyme method.Total sugar contents in each fraction were 70.45% and 84.86%,respectively.Meanwhile,YPⅠwas mostly mannose, and β-1,3-D-glucan of YPⅡwas in high purity.
Pages: 151-153
Year: 2013 Issue: 5
Journal: Food & Machinery
Keyword: yeast cell wall; β-1; 3-D-glucan; alkali-enzyme method; structure;
Abstract: Studied the method of extraction and separation of β-1,3-Dglucan from yeast and identified its structure.The yeast polysaccharide was extracted by alkali-enzyme method,then,the structure of yeast glucan was analyzed by IR,and its monosaccharide compositions were analyzed by HPLC.Two fractions of polysaccharides were isolated and identified as YPⅠand YP,Ⅱrespectively,by alkali-enzyme method.Total sugar contents in each fraction were 70.45% and 84.86%,respectively.Meanwhile,YPⅠwas mostly mannose, and β-1,3-D-glucan of YPⅡwas in high purity.
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