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Cloning and sequence analysis of VP1 gene fragment from a strain of infectious flacherie virus isolated in Zhejiang, China
Author(s): 
Pages: 1016-1021
Year: Issue:  10
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  家蚕传染性软化病病毒VP1基因片段结构蛋白克隆序列分析;
Abstract: 以首株在中国分离到的家蚕传染性软化病病毒(Bombyx mori infectious flacherie virus,BmIFV)BmIFV-CHN001基因组为模板,扩增了编码主要结构蛋白的VP1基因.克隆测序后得到VP1基因片段906 bp.该序列与已发表的日本毒株相比,核苷酸序列的相似性为99.3%,编码氨基酸的相似性为100%,证明该毒株与家蚕传染性软化病病毒日本株的同源性较高.把BmIFV-CHN001的VP1序列与同属的另外6个昆虫小RNA病毒的结构蛋白进行序列比对,构建系统发育树,对其进化关系进行了初步分析,结果显示这7种病毒具有相近的亲缘关系,而BmIFV-CHN001与蜜蜂囊雏病毒的亲缘关系最近.
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