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Bioactivity of rhodojaponin-Ⅲ on Spodoptera litura cells and its mechanism
Author(s): 
Pages: 1022-1026
Year: Issue:  10
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  斜纹夜蛾闹羊花素Ⅲ生物活性;
Abstract: 研究了闹羊花素Ⅲ对斜纹夜蛾Spodoptera litura离体培养细胞(SL细胞)的活性,并测定了对SL细胞Na+、K+和葡萄糖吸收以及对4龄幼虫血细胞数量的影响.结果表明:以400 μg/mL和200 μg/mL闹羊花素Ⅲ处理SL细胞,24 h后细胞的相对死亡率为79.00%和56.69%,处理后8 h,16 h,24 h和48 h的LC50分别为240.09 μg/mL,173.45μg/mL,113.56 μg/mL和73.40 μg/mL;闹羊花素Ⅲ处理SL细胞后10 min,细胞对离子的吸收迅速增加,30 min后吸收作用逐渐减弱;处理后3天内细胞对葡萄糖的吸收迅速增加,4~5天后,细胞对葡萄糖的吸收基本停止.以叶碟法和注射法处理4龄幼虫,8 h后幼虫血细胞数量显著降低,随处理时间增加,幼虫血细胞数量又逐渐增加.
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