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Pages: 21-28
Year: Issue:  2
Journal: Journal of Nanjing Forestry University(Natural Sciences Edition)

Keyword:  薄层层析分离生物鉴定抑制物质抑制效应乙醇浸提紫外光谱细胞分裂素天然激素化学鉴定水稻幼苗;
Abstract: The purpose of this research is to study the natural hormones in the sprouts of Cunninghamia lanceolata (Lamb.) Hook. Samples were partitioned in solvents and isolated by cation exchange resins and DEAE-cellulose columns. The extracts were purified by paper and thin-layer chromatography and the fractions were verified by means of bioassay, with wheat coleoptile section being adopted for IAA and ABA, rice seedling for GAs, and radish cotyledon for cytokinins respectively. The results showed that there existed in the extracts only a little auxin and growth inhibitors but quite some GAs and cytokinins, with the activities of cytokinins being most prominent.By TLC on silica gel GF254 and using the solvent butanol-isopropanol-ammonia-water (2:8:1:1) for the separation of cytokinins, six dark spots appeared in the UV light. The spots were eluted with 90% ethanol separately. The eluates were examined with a spectrophotometer. The ultraviolet absorptive spectra showed that four eluates had weak absorptive peaks at 270 nm, and the other two eluates showed high peaks at 260 nm, all of them having biological activities of cytokinins. From this experiment we may conclude that the sprouts are rich in cytokinins, which might be an important factor causing the buds to have high vigor for sprouting.
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