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cDNA Cloning and Sequence Analysis of the Mature Inhibin/Activin βA Subunit from Xianju Chicken
Author(s): NIU Dong, RUAN Zuo, FU Yan, YU Xuping, YANG Peixin
Pages: 3-
4
Year: 2002
Issue:
3
Journal: CHINESE JOURNAL OF ANIMAL SCIENCE
Keyword: 仙居鸡; 抑制素/活化素βA亚基; cDNA克隆; 序列分析;
Abstract: 本研究根据发表的来航鸡抑制素/活化素βA亚基序列设计引物,运用RT-PCR技术从仙居鸡卵泡的颗粒细胞总RNA中扩增出抑制素/活化素βA亚基成熟区序列,并进行了克隆和测序.结果显示,所测鸡成熟βA亚基是由116个氨基酸(aa)残基组成的蛋白质,共有9个半胱氨酸残基,与发表的鸡及哺乳类相应序列对比,其核苷酸序列的同源性分别为99.2%和81%~85.2%,其预测氨基酸序列的同源性分别为100%和96.6%~97.4%,且所测鸡βA亚基成熟区半胱氨酸残基的数目和位置与发表的鸡和哺乳类相同,说明该亚基的序列及结构在不同物种间具高度保守性,揭示其可能具重要的生理功能.
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