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Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage
Pages: 297-303
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  Subarachnoid hemorrhageHydrogen sulfideS-100bB-cell lymphoma-2c-FosMatrixmetalloproteinase-9ImmunofluorescenceRat;
Abstract: Objective To explore the effect of hydrogen sulfide( H2S) on the brain injury in rats with subarachnoid hemorrhage( SAH). Methods Thirty experimental rats were randomly divided into control group( n = 10),SAH model group( n = 10) and Na HS( 100 mg / kg) group( n = 10). The experimental rats of the H2 S group were injected with Na HS 100 mg / kg body weight in intraperitoneal and after surviving for 24 hours to do the neurological assessment and took the whole brain. The expression changes of GFAP,S-100 b,B-cell lymphoma-2( Bcl-2), c-Fos, Caspase-3,matrixmetalloproteinase-9( MMP-9) were detected with immunofluorescence and Western blotting method. Results Neurological assessment showed that severe neurological symptoms were significantly relieved in hydrogen sulfide group.GFAP and S-100 b had a high expression in SAH rats group and were significantly decreased in H2 S pretreatment group.The expression of Bcl-2 and Caspase-3 in SAH group were high than that in the control group but the expression of Bcl-2was increased in H2 S pretreatment group than SAH group,and Caspase-3 was decreased in H2 S pretreatment group.Western blotting analysis showed that the expression of c-Fos and MMP-9 were increased in SAH group and was significantly decreased in H2 S pretreatment group. Conclusion The hydrogen sulfide has an significant protective effect onbrain injury after SAH and its mechanism may be improved the function of glial cells and apoptotic pathways.
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