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Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine
Pages: 379-386
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  Angelica sinensis polysaccharideEphedrineLiver histological structureBax proteinCaspase-3 proteinImmunohistochemistryMouse;
Abstract: Objective To investigate the protection of Angelica sinensis polysaccharide( ASP) on ephedrine induced hepatic injury in mice. Methods Seventy-two mice were randomly divided into 4 groups: the natural control group,experiment control group,ASP1 group,and ASP2 group. The experimental control group and ASP groups were intraperitoneally injected with a 4. 0g / L of ephedrine 0. 2ml twice a day for 15 days,and the natural control group was injected with a same amount of saline. The ASP groups were irrigated to stomach with 0. 3ml Angelica sinensis polysaccharide solution( 12. 5g / L,25. 0g / L) 1 hour after ephedrine injection,the natural control group and experiment control group were irrigated to stomach with a same amount of saline. The mice were sacrificed at 5 day,10 day,and 15 day after treatment,respectively. The morphological observation was used to observe pathological changes of hepatic tissues. Activities of hepatic histologicalsuperoxide dismutase( SOD) and plasma γ-glutamyltransferase( γ-GT),alkaline phosphatase( AKP),glutamicpyruvic transaminase( ALT),glutamic-oxalacetic transaminase( AST),as well as content of malonaldehyde( MDA) were detected by colorimetry. The expressions of Bax protein and Caspase-3 protein were measured by immunohistochemistry in liver of mice. Results There were various degree damages in hepatic tissues,hepatic cords were disorganized,liver cells were loose and liver sinusoid expanded,the activity of γ-GT、AKP、ALT、AST in plasma were significantly increased,the activity of SOD in liver tissue was significantly reduced,the content of MDA in liver tissue was increased significantly,the expression of Bax and Caspase-3 protein in liver were significantly increased. Compared with the experimental control group,pathological damage of liver was significantly improved,the activity of plasma γ-GT,AKP,ALT,AST were significantlyreduced,the activity of SOD in liver tissue was increased significantly,however the content of MDA was significantly decreased and the expressions of Bax and Caspase-3 protein in liver were reduced in Angelica sinensis polysaccharide group.Conclusion Angelica sinensis polysaccharide can improve liver tissue antioxidant capacity and promote reparation of cell injury and protect hepatic injury which is induced by ephedrine.
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