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Protective effects of Shandan Sphallerocarpus gracilis polysaccharide on chronic hepatic injury in mice induced by CCl4
Pages: 387-393
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  Shandan Sphallerocarpus gracilis polysaccharideCarbon tetrachlorideLiverTissue structureSuperoxide dismutaseColorimetric methodMouse;
Abstract: Objective To investigate the effect of Shandan Sphallerocarpus gracilis polysaccharide( SGP) on the hepatic injury in mice induced by carbon tetrachloride( CCl4). Methods Forty mice were divided into 5 groups: the nature control group,the experimental control group,and 3 SGP groups. The mice in SGP groups were administered with SGP at the doses of 12. 5,25,37. 5 g / L 0. 2ml,respectively,the other groups were gavaged with 0. 2ml saline,the nature control group was infected by 0. 2ml injected by 0. 2ml saline. The morphological changes for hepatic tissues were observed under microscopy,the activities of plasma alanine aminotransferase( ALT),aspartate aminotransferase( AST),alkaline phosphatase( ALP) and superoxide dismutase( SOD),as well as content of malondialdehyde( MDA) in liver were determined by colorimetry,the expression change of Caspase-3 and Bax in liver tissue was determined by the immunohistochemical method. Results The activities of plasma ALT,AST,ALP was significantly increased in the experimental control group,compared with the nature control group( P < 0. 01),while the activity of SOD in liver tissue decreased obviously( P < 0. 01),and the contents of MDA were increased significantly( P < 0. 01),the livers tissue structure in experimental control group was blur,liver cells were necrosis and had serious vacuolation. The activities for Caspase-3and Bax immunohistochemical positive staining in hepatic tissues significantly increased in the experimental control group( P < 0. 01). However,the activities of plasma ALT,AST and ALP in the SGP groups was significantly decreased( P <0. 01),the activitys of SOD in liver tissue were increased significantly( P < 0. 01),and the contents of MDA were significantly decreased( P < 0. 01),the liver structures were normal,inflammatory necrosis were decreased,liver celluarstuctures were clear,the positive expression of Caspase-3 and Bax protein were decreased significantly,compared with the experimental control group( P < 0. 01). Conclusion The SGP has a good effect on improving the cellular antioxidant activity,exhibiting activities of anti-oxidant and anti-inflammatory,and protecting the liver from the damage induced by carbon tetrachloride in mice,and has a dose effect within a certain range.
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