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Change of apoptosis factor expression in cochlear nucleus in carboplatin-induced auditory neuropathy rat
Pages: 324-328
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  Cochlear nucleusAuditory neuropathyImmunohistochemistryRT-PCRRat;
Abstract: Objective To investigate the influence of carboplatin( CBP) on cell apoptosis in the cochlear nucleus and the nerve protection of dexamethasone( DEX) in rats. Methods CBP was used to establish the animal model of auditory neuropathy( AN) through an intraperitoneal injection. Rats were assigned to NS group,CBP group and DEX +CBP group,18 rats per group. On day 3 and day 6 post modeling,the immunoreactivity of Caspase-3 in the cochlear nucleus was detected by immunohistochemistry and the apoptotic protease activating facter 1( Apaf-1) mRNA expression was explored by RT-PCR. Results The level of immunoreactivity of Caspase-3 and expression of Apaf-1 mRNA in the ventral cochlear nucleus( VCN) and dorsal cochlear nucleus( DCN) of CBP group were significantly higher than those in NS group and DEX + CBP group( P < 0. 05),while the levels between NS group and DEX + CBP group had no significant differences( P > 0. 05),and the effect on day 6 was more obvious than that on day 3. Conclusion CBP can not only damage the auditory nerve but also cause cell apoptosis on the cochlear nucleus in rats,and DEX weakens the effect of apoptosis for its neuroprotection.
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